Utilizing 30 ethnically diverse human subjects, the Hand Sanitizer Lotion with Invisicare was applied to the volar forearm, and then scrubbed with gauze saturated with 5% Ammonium Hydroxide at intervals of 10 minutes, 2 hours and 4 hours. The same gravimetric analysis as used in the in vitro studies, were performed on the skin wash solutions. Throughout the four-hour test period, punch biopsies were harvested from random subjects for examination under Electron Microscopy and Atomic Force Microscopy.